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目的:通过HPLC建立九香虫药材特征图谱，并测定尿嘧啶、腺嘌呤、尿苷、尿酸、次黄嘌呤、腺苷、黄嘌呤和犬尿喹啉酸8个核苷类成分含量，为其质量控制及评价提供参考。方法:采用Agilent ZORBAX SB-Aq色谱柱（4.6 mm×250 mm，5 μm），以甲醇（A）-0.05%磷酸溶液（B）为流动相，梯度洗脱，柱温25 ℃，流速0.8 ml/min，检测波长254 nm，建立九香虫药材HPLC特征图谱，并对8个指标成分含量进行测定。结果:建立了15批九香虫药材特征图谱，确定10个共有特征峰，并指认其中8个，各样品特征图谱与对照图谱相似度在0.969～0.997之间。含量测定结果表明，尿嘧啶、腺嘌呤、尿苷、尿酸、次黄嘌呤、腺苷、黄嘌呤和犬尿喹啉酸线性范围分别为0.002 0～0.644 0 μg、0.001 4～0.448 0 μg、0.001 0～1.257 0 μg、0.005 4～6.221 0 μg、0.001 0～0.724 0 μg、0.001 0～0.644 0 μg、0.002 0～1.113 0 μg、0.003 8～2.059 0 μg，线性关系良好（ r≥0.999）；重复性和稳定性 RSD均<2.0％，平均加样回收率在99.36%～103.40％之间。 结论:本研究建立的特征图谱及核苷类成分含量测定方法简便、可靠、重复性好、精密度高，可用于九香虫药材的定性、定量分析，可为九香虫质量评价提供参考。

Objective:To establish HPLC chromatogram for Aspongopus; To determine 8 nucleoside components of uracil, adenine, uridine, uric acid, hypoxanthine, adenosine, xanthine and canine quinolinic acid; To provide reference for quality control and evaluation.Methods:The Agilent ZORBAX SB-Aq chromatographic column (4.6 mm×250 mm, 5 μm) was used for gradient elution with mobile phases consisting of a methanol (A) and 0.05% phosphoric acid (B). The column temperature was 25 ℃, the flow rate was 0.8 ml/min, and the detection wavelength was 254 nm. HPLC chromatograms for Aspongopus were established and the contents of 8 components were determined.Results:The characteristic chromatogram of 15 batches aspongopus herbs was established. A total of 10 common characteristic peaks were identified and 8 were identified. The similarity between the characteristic chromatogram of samples and the control chromatogram was 0.969-0.997. The content determination showed that the linear range of uracil, adenine, urin, uric acid, hypoxanthine, adenosine, xanthine and xanuric acid was among 0.002 0-0.644 0, 0.001 4-0.448 0, 0.001 0-1.257 0, 0.005 4-6.221 0, 0.001 0-0.724 0, 0.001 0-0.644 0, 0.002 0-1.113 0, 0.003 8-2.059 0 μg, respectively, with a good linear relationship ( r≥0.999); the repeatability and stability of RSD were <2.0%, and the average sampling recovery rate was between 99.36% and 103.40%. Conclusion:The characteristic chromatogram and content determination method established in this study are simple, reliable, reproducible and accurate, and can be used for the qualitative and quantitative analysis of Aspongopus and can provide a reference for the quality evaluation method of the Aspongopus.