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目的:探讨补中益气汤对A549细胞荷瘤裸鼠移植瘤中多药耐药相关蛋白（MRP）、肺耐药蛋白（LRP）、P-糖蛋白（P-gp）表达的影响。方法:培养A549细胞建立荷瘤裸鼠移植瘤模型，按随机数字表法分为空白对照组、顺铂组及顺铂联合补中益气汤低、中、高剂量组。相应药物干预25 d后，采用RT-qPCR测定移植瘤MRP、LRP、P-gp mRNA水平，Western Blot检测移植瘤组织MRP、LRP、P-gp蛋白表达情况。结果:与空白对照组比较，顺铂组MRP、LRP、P-gp mRNA及蛋白表达降低（ P<0.05）；与顺铂组比较，顺铂联合补中益气汤低、中、高剂量组MRP、LRP、P-gp mRNA水平及蛋白表达降低（ P<0.05）。 结论:补中益气汤可抑制A549细胞荷瘤裸鼠移植瘤MRP、LRP、P-gp表达，且无剂量依赖性。

Objective:To investigate the effects of Buzhong Yiqi Decoction on the expressions of multidrug resistance-related protein (MRP), lung resistance-related protein (LRP), and P-glycoprotein (P-gp) in transplantation tumor of A549 tumor-bearing nude mice.Methods:A549 cells were cultured to establish xenograft models of tumor-bearing nude mice. According to the random number table method, they were divided into blank control group, cisplatin group, cisplatin + Buzhong Yiqi Decoction low-dosage group, cisplatin + Buzhong Yiqi Decoction medium-dosage group, cisplatin + Buzhong Yiqi Decoction high-dosage group. After 25 days of corresponding drug intervention, the mRNA expressions of MRP, LRP and P-gp in transplantation tumors were detected by Real-Time PCR, and Western blot was used to detect the expressions of MRP, LRP and P-gp proteins in xenograft tissues in each group.Results:Compared with blank control group, the mRNA and protein expressions of MRP, LRP and P-gp in cisplatin group were significantly down-regulated ( P<0.05). Compared with cisplatin group, the mRNA and protein expressions of MRP, LRP and P-gp in the cisplatin + Buzhong Yiqi Decoction low-dosage group, cisplatin + Buzhong Yiqi Decoction medium-dosage group, cisplatin+Buzhong Yiqi Decoction high-dosage group decreased ( P<0.05). Conclusion:Buzhong Yiqi Decoction can inhibit the expressions of MRP, LRP and P-gp in transplantation tumor of A549 tumor-bearing nude mice and without dosage dependence.