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目的 建立简便有效的巴马小型猪肢体缺血再灌注模型.方法 将12只巴马小型猪采用随机数字表法分为对照组(Sham)和肢体缺血再灌注损伤组(LI/RI),每组6只.Sham组仅暴露血管,LI/RI组夹闭血管并缚扎止血带模拟损伤.比较两组的氧化应激、炎性反应和组织损伤指标,采用两因素重复测量方差分析和t检验.结果 7 d后成功构建模型.LI/RI组血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、肌酸激酶MB同工酶(CK-MB)、谷草转氨酶(AST)、谷丙转氨酶(ALT)指标整体高于Sham组(F=8.554,P＜0.01;F=9.167,P＜0.01;F=3.639,P＜0.05;F=10.750,P＜0.01;F=100.336,P＜0.01;F=118.641,P＜0.01;F=5.219,P＜0.05),血清超氧化物歧化酶(SOD)指标整体低于Sham组(F=2.471,P＜0.05).LI/RI组肌肉MDA、TNF-α,心肌MDA指标和肝脏MDA、TNF-α、IL-6指标高于Sham组[(1.93±0.60)nmol/mg prot 比(0.95±0.24)nmol/mg prot,t=-4.666,P＜0.01;(75.61±44.17)pg/ml 比(20.65±8.91)pg/ml,t=-2.648,P＜0.05,(2.56±1.03)nmol/mg prot 比(1.20±0.40)nmol/mg prot,t=-3.192,P＜0.05;(2.88±0.76)nmol/mg prot 比(0.80±0.28)nmol/mg prot,t=-6.218,P＜0.05;(1 080.79±256.78)pg/ml 比(441.08±135.50)pg/ml,t=-4.995,P＜0.01;(209.39±94.61)pg/ml 比(37.18±32.79)pg/ml,t=-3.479,P＜0.05],LI/RI 组肌肉、心肌 SOD 指标低于 Sham 组[(87.64±15.13)U/mg prot 比(114.56±10.41)U/mg prot,t-3.006,P＜0.05;(752.26±118.97)U/mg prot 比(939.73±90.79)U/mg prot,t=2.777,P＜0.05].LI/RI组的光镜下骨骼肌、心脏和肝脏出现明显损伤.结论 6 h夹闭血管联合止血带可成功建立LI/RI模型,引起肌肉损伤、全身氧化应激、炎性反应及心脏、肝脏损伤.

Objective To establish a simple and effective limb ischemia-reperfusion injury(LI/RI)model in Bama miniature pigs.Methods Totally,12 Bama miniature pigs were randomly divided into a control group(Sham,n=6)and a LI/RI group(n=6),with 6 pigs in each group.The Sham group underwent exposure of blood vessels only,nd the LI/RI group had blood vessels clamped and a tourniquet applied to simulate injury.Oxidative stress,inflammatory response,and tissue injury indicators were com-pared between the two groups using two-way repeated measures ANOVA and t-tests.Results After 7 days,the model was successfully established.The LI/RI group showed significantly higher levels of serum crea-tine kinase(CK),lactate dehydrogenase(LDH),malondialdehyde(MDA),tumor necrosis factor-alpha(TNF-α),creatine kinase-MB(CK-MB),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)than the Sham group(F=8.554,9.167,3.639,10.750,100.336,118.641,5.219,P＜0.05),and lower levels of superoxide dismutase(SOD)(F=2.471,P＜0.05).The LI/RI group had higher levels of muscle MDA,TNF-α,myocardial MDA,and hepatic MDA,TNF-α,IL-6 than the Sham group[(1.93±0.60)nmol/mg prot vs.(0.95±0.24)nmol/mg prot,t=-4.666,P＜0.01;(75.61±44.17)pg/ml vs.(20.65±8.91)pg/ml,t=-2.648,P＜0.05;(2.56±1.03)nmol/mg prot vs.(1.2±0.40)nmol/mg prot,t=-3.192,P＜0.05;(2.88±0.76)nmol/mg prot vs.(0.80±0.28)nmol/mg prot,t=-6.218,P＜0.05;(1 080.79±256.78)pg/ml vs.(441.08±135.50)pg/ml,t=-4.995,P＜0.01;(209.39±94.61)pg/ml vs.(37.18±32.79)pg/ml,t=-3.479,P＜0.05],and lower levels of muscle and myocardial SOD than the Sham group[(87.64±15.13)U/mg prot vs.(114.56±10.41)U/mg prot,t=3.006,P＜0.05;(752.26±118.97)U/mg prot vs.(939.73±90.79)U/mg prot,t=2.777,P＜0.05].Histological examination revealed more severe damage to the skeletal muscle,heart,and liver in the LI/RI group.Conclusion The 6-h vascular clamping combined with a tourniquet can successfully establish the LI/RI model,causing muscle injury,systemic oxidative stress,inflammatory response,and damage to the heart and liver.