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Expression and Clinical Significance of miR-23a and Metastasis Suppressor 1 From Tissue of Gastric Adenocarcinoma
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- Author:
- No author available
- Journal Title:
- JOURNAL OF RADIOIMMUNOLOGY
- Issue:
- 6
- DOI:
- 10.3969/j.issn.1008-9810.2013.06.047
- Key Word:
- 胃肿瘤;肿瘤抑制蛋白质类;原位杂交;免疫组织化学
Abstract： 目的:探讨miR-23a与转移抑制因子1(MTSS1)在胃腺癌组织中的表达及其临床意义.方法:运用荧光素报告载体系统检测miR-23a直接调控的靶基因并采用Transwell侵袭实验检测miR-23a对人胃腺癌细胞的侵袭能力.收集胃腺癌患者手术标本46例,癌旁组织作为正常对照,分别运用原位杂交,免疫组织化学EnVision法检测胃腺癌组织及癌旁组织中miR-23a,MTSS1的表达水平,并对二者进行相关性分析.结果:MTSS1是miR-23a直接调控的靶基因,miR-23a下调MTSS1蛋白的表达,增强胃癌细胞的侵袭能力.在46例胃腺癌组织中miR-23a阳性表达率为87.0%(40∕46),MTSS1蛋白阳性表达率为17.4%(8∕46),分别与癌旁对照组比,差异有统计学意义(P<0.01);miR-23a的表达随胃腺癌临床分期演化(P<0.05)和浸润深度增加(P<0.01),且有淋巴结转移的miR-23a的表达显著高于无淋巴结转移组(P<0.05);MTSS1的表达随胃腺癌临床分期演化(P<0.05)和浸润深度增加而下调(P<0.05),且有淋巴结转移的MTSS1的表达显著低于无淋巴结转移组(P<0.01);相关性分析表明,miR-23a表达与MTSS1的表达呈显著负相关(r=-0.594,P<0.05).结论:miR-23a通过靶向抑制MTSS1促进胃腺癌细胞生长,侵袭转移;miR-23a的高表达和MTSS1蛋白低表达可能是胃腺上皮恶性转化以及胃癌发生浸润转移的重要生物学标志,检测二者对预测胃癌侵袭及转移有重要意义.
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