Abstract： Objective To explore the differential expression proteins in uterine myometrial tissues of adenomyosis using TMT quantitative proteomics technology and to analyze the differential signaling pathways using bioinformatics.Methods Uterine myometrial tissues from 30 patients with adenomyosis and 30 patients without adenomyosis were col-lected.TMT quantitative proteomics technology was used to study the differential expression proteins between the two groups.Bioinformatics analysis of the differential expression proteins was performed using GO and KEGG analysis soft-ware.Western blot was used to validate the selected differential expression proteins.Results A total of 62 differentially expressed proteins were identified using TMT proteomics technology,including 7 up-regulated proteins and 55 down-regulated proteins.KEGG enrichment analysis showed that various immune signaling pathways and calcium signaling path-ways were associated with adenomyosis.Finally,three significantly up-regulated differential expression proteins(EIF4G1,CAPN2,ALG13)and three significantly down-regulated differential expression proteins(Clusterin,GAS1,SAP)were selected for Western blot validation,and the results were consistent with the proteomic results,with statistical-ly significant differences(P＜0.05).Conclusion TMT quantitative proteomics technology identified multiple differenti-ally expressed proteins in adenomyosis and revealed their involvement in various immune signaling pathways,providing re-search directions for the pathogenesis and treatment targets of this disease.