Abstract： Objective To evaluate the antioxidant effect of American ginseng Rhodiola formula on ethanol-induced oxidative damage in model mice,and to explore its mechanism of action,providing a scientific basis for the development of healthy food.Methods Mice were randomly divided into blank control group,acute oxidative injury model group,low dose(330 mg/kg),medium dose(660 mg/kg)and high dose(990 mg/kg)groups according to body weight,10 mice in each group.Each dose group was given test samples by oral gavage.Blank control group and model group were given equal volume of distilled water.After continuous gavage for 30 days,50%ethanol was intragastrically administered at12 mL/kg after fasting for 16 h in each group except blank control group.Blood and liver were collected from mice 6 h later.Malondialdehyde(MDA),8-epoxyisoprostane(8-Isoprostane),protein carbonyl(PCO),glutathione(GSH),aspartate aminotransferase(AST),alanine aminotransferase(ALT),triglyceride(TG),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)content and superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity in mouse serum,MDA,PCO,GSH content and SOD activity in liver tissue were measured,and the protein expression of p-p38,p-ERK and p-JNK was detected by Western blotting..Results Compared with the model group,the MDA content in the ser-um of mice in the low-dose group of American ginseng Rhodiola formula decreased(P<0.05),the 8-Isoprostane and PCO contents in each dose group decreased(P<0.05),the GSH content and GSH-Px and SOD activities in each dose group increased(P<0.05),the TNF-α,IL-1β,and IL-6 levels in each dose group decreased(P<0.05),and the ALT and AST activities in each dose group de-creased(P<0.05).The MDA content in the liver of mice in the low and medium dose groups decreased(P<0.05),the PCO and TG contents in each dose group decreased(P<0.05),and the SOD activity in the low and medium dose groups and the GSH content in the low dose group increased(P<0.05).The low dose group could inhibit the expression of p-p38 and p-JNK(P<0.05),and the medium and high dose groups could inhibit the expression of p-p38,p-ERK and p-JNK(P<0.05).Conclusion Rhodiola rosea,American ginseng,acerola cherry powder and grape seed extract composition of medicinal food homologous complex formula has a strong antioxidant health care effect.