Abstract： Objective To study the impact of acute blood glucose fluctuation on the level of N-terminal pro-brain natriuretic peptide(NT-proBNP).Methods Totally 32 inpatients undergoing oral glucose tolerance tests(OGTT)from September to October 2019 were randomly selected.Plasma NT-proBNP,blood glucose,insulin and C-peptide levels were measured at the fasting state,and 30-minute,60-minute,120-minute and 180-minute following OGTT.The correlation between individual NT-proBNP level and blood glucose concentration was assessed by Pearson's and Spearman's correlation coefficients.The one-way analysis of variance(ANOVA)and generalized linear mixed model(GLMM)were applied to further analyze the effects of the detection time of blood glucose,blood glucose level,insulin and C-peptide on NT-proBNP level.Results Correlation analysis showed that a significant correlation between NT proBNP level and blood glucose concentration was only found in 5 cases out of 32 patients(P＜0.05).However,the correlation trends and best fit curves between NT proBNP level and blood glucose concentration varied in all the patients.One-way ANOVA did not identify a significant difference in NT proBNP level at different time points and different blood glucose concentrations(F[1.36,40.87]=1.23,P=0.29,η2=0.04).GLMM showed that NT-pro-BNP level was not impacted by the detection time of blood glucose,blood glucose level,insulin and C-peptide(P＞0.05).Conclusion Acute blood glucose fluctuation does not impact the level of NT-proBNP,and thereby it does not impact the evaluation on cardiac function.