Abstract： Objective Observe the inhibitory effect of dihydrocapsaicin on hepatocellular carcinoma cell(HCC)HepG2 cells and explore the molecular mechanism.Method Divided into blank group and dihydrocapsaidine(100μmol/L,150μmol/L,200μmol/L,250μmol/L)group,Cell counting(CCK-8)was used to detect the activity and quantity of HepG2 cells at different time(24h,48h,72h)and different concentrations of dihydrocapsaicin(100μmol/L,150μmol/L,200μmol/L,250μmol/L);Using fluorescence quantitative PCR(RT-PCR)of different concentrations of dihydrocapsaicine(150μmol/L,200μmol/L,250μmol/L)on HepG2 cells for 24h,The expression level of intracellular apoM mRCR;After immunosorbent assay(ELISA),different concentrations(150μmol/L,200μmol/L,250μmol/L)on HepG2 cells,The apoM levels within the cell supernatant.Results Compared to the blank group,Concentration of dihydrocapsaicin at 100μmol/L,No statistically significant differences at each time(24h,48h and 72h),The remaining concentrations of dihydrocapsaicin(150μmol/L,200μmol/L,250μmol/L)were statistically significant at each time(24h,48h,72h),It suggests that HepG2 cells have certain time-and concentration-dependent effects on dihydrocapsaicin;Compared to the blank group,In the presence of dihydrocapsaicin at concentrations of 150μmol/L,200μmol/L,and 250μmol/L,The marked downregulation of apoM mRNA expression levels within HepG2 cells in HCC,Moreover,the apoM level in the supernatant of HCC HepG2 cells also decreased(P<0.05).Conclusion Dihydrocapsaicin has an inhibitory effect on HCC HepG2 cells,and its inhibitory effect may be related to the inhibition of apoM expression in HCC HepG2 cells.