Abstract： Objective To explore the clinical effect of a new type of fractional CO2 laser combined with neuromuscular stimulation in the treatment of postpartum vaginal relaxation and its impact on vaginal relaxation,pelvic floor muscle function and sexual function.Methods A total of 68 patients with postpartum vaginal relaxation treated were retrospectively analyzed.According to the treatment plan,there were 34 cases in the control group(neuromuscular stimulation therapy)and 34 cases in the study group(new fractional CO2 laser combined with neuromuscular stimulation therapy).The vaginal relaxation,pelvic floor muscle voltage(class Ⅰ muscle fibers,class Ⅱ muscle fibers),pelvic floor muscle strength(resting,fast and slow muscles),sexual function,vaginal mucosal indexes and vaginal relaxation related indexes[serum transforming growth factor-β1(TGF-β1),relaxant(RLX),estradiol(E2)]were statistically compared between the two groups before and after treatment.Results Com-pared with the control group,the vaginal relaxation in the study group was decreased after treatment(P<0.05);compared with the control group,the voltage of type Ⅰ muscle fiber and type Ⅱ muscle fiber in the study group were increased after treatment(P<0.05);compared with the control group,the resting muscle strength in the study group was decreased,while the fast and slow muscle strength were increased after treatment(P<0.05);compared with the control group,the scores of of vari-ous dimensions of sexual function in the study group were increased after treatment(P<0.05);compared with the control group,the scores of various dimension of vaginal mucosa index in the study group were increased after treatment(P<0.05);compared with the control group,the levels of serum TGF-β1 and E2 in the study group were increased after treatment,while the level of RLX was decreased(P<0.05).Conclusion The new fractional CO2 laser combined with neuromuscular stim-ulation in the treatment of postpartum vaginal relaxation can effectively reduce vaginal relaxation,improve pelvic floor muscle function,improve sexual function,vaginal mucosal secretion,and promote local tissue repair.