Abstract： Objective:To explore the best preparation method of permanent slide sealed specimen of parasite egg fluid.Methods:On the basis of the existing research,the preservation formula of egg suspension was improved,and egg slide specimens were prepared with different sealing methods.After one year of preservation,the production effect was observed.Results:The eggs were preserved with 50％volume egg white,40％volume formalin and 10％volume glycerin,and the best results were obtained by paraffin ring and gum double sealing tablets.Conclusion:It is found that the content of glycerin in egg preservation solution has a great influence on the effect of permanent sealing of egg slide specimen,and the sealing method is particularly important for the long-term preservation of egg solution.