Abstract： Objective To investigate the diagnostic value of microribonucleic acid-365(miR-365)and chemokine ligand 18(CCL-18)in endometrial cancer(EC)and its relationship with clinical pathological features.Methods A total of 214 EC patients diagnosed in author's hospital from December 2018 to December 2021 were collected as the EC group,while 212 patients with benign endometrial diseases at the same period were selected as the control group.The expression level of miR-365 in serum and cancer tissues was detected by reverse transcription polymerase chain reaction(RT-PCR),the serum CCL-18 level was detected by enzyme linked immunosorbent assay,and the CCL-18 level in cancer tissues was detected by immunohistochemical method.EC patients were divided into miR-365 high expression group(n=106)and miR-365 low expression group(n=108)based on the median expression level of miR-365 in cancer tissues;meantime,according to the expression of CCL-18 in cancer tissues,the patients were divided into CCL-18 negative expression group(n=66)and CCL-18 positive expression group(n=148).The diagnostic values of serum miR-365 and CCL-18 levels for EC were analyzed by receiver operating characteristic(ROC)curve.Results The serum CCL-18 level in the EC group was higher than control group,while the miR-365 level was lower than control group(all P＜0.05).The positive expres-sion rate of CCL-18 in cancer tissues of EC patients was higher than that in adjacent tissues,and the level of miR-365 was lower than that in adjacent tissues(all P＜0.05).The proportion of EC patients with clinical stage Ⅲ-Ⅳ,low differenti-ated tissues,lymph node metastasis and infiltration depth ≥ 1/2 in miR-365 low expression group and CCL-18 positive ex-pression group were higher than those in miR-365 high expression group and CCL-18 negative expression group(all P＜0.05).The area under the curve(AUC)of serum miR-365,CCL-18 and their combined diagnosis for EC was 0.718,0.791 and 0.846 respectively.Conclusion miR-365 is lowly expressed in the serum and cancer tissues of EC patients,while CCL-18 is highly expressed,the combination of the two can effectively diagnose EC,which can provide reference for early clinical diagnosis of EC.