Abstract： Objective 45 batches of Ligusticum Sinense from different origins were collected to establish high performance liquid chromatography fingerprint,and combine fingerprint similarity evaluation,cluster analysis,principal component analysis,and partial least squares discriminant analysis to evaluate the quality of Ligusticum Sinense from different origins,in order to achieve more comprehensive drug quality control.Methods According to the 2020 edition of the Chinese Pharmacopoeia,the quality evaluation of 45 batches of different raw materials of Ligusticum Sinense was conducted using methods such as moisture and total ash determination,acid insoluble ash determination,alcohol soluble extract determination,and content determination.At the same time,high performance liquid chromatography was used to establish fingerprint spectra of different original Ligusticum Sinense,and the similarity evaluation system of traditional Chinese medicine fingerprint spectra was used to evaluate their similarity.SPSS software was used for significance difference analysis,cluster analysis,principal component analysis,and SIMCA was used for partial least squares discriminant analysis.Results The determination results of water content,ash content,and acid insoluble ash content of different types of Ligusticum Sinense meet the requirements of the pharmacopoeia.The average content of Ligusticum Sinense extract is 37.59%,that of Ligusticum Jeholense Nakai et Kitag.extract is 19.59%,and that of Conioselinum Vaginatum(Spreng.)is 14.75%.The average content of ferulic acid in Ligusticum Sinense is 0.07%;the average content of ferulic acid in Ligusticum Jeholense Nakai et Kitag.is 0.09%;the average content of ferulic acid in Conioselinum Vaginatum(Spreng.)was 0.13%.The similarity evaluation results of the fingerprint spectra showed that the similarity of the fingerprint spectra of the same original Ligusticum Sinense is between 0.973 and 0.999,while the similarity between Ligusticum Sinense and Ligusticum Jeholense Nakai et Kitag.was between 0.612 and 0.780,and the similarity between Ligusticum Sinense and Conioselinum Vaginatum(Spreng.)was between 0.108 and 0.212.Through cluster analysis,different Ligusticum Sinense could be successfully classified into three categories.Through principal component analysis for comprehensive evaluation,Conioselinum Vaginatum(Spreng.)had a higher comprehensive score.Through PLS-DA analysis,it was predicted that vanillin,ferulic acid,and ligustilide might be the main reasons for differences in the quality of different Ligusticum Sinense.Conclusion There are certain differences in extract content,ferulic acid content,and fingerprint among different types of Ligusticum Sinense,which can provide a basis for clinical drug selection,new drug development,and quality control of Ligusticum Sinense.