Abstract： Objective To study the protective effect of Qingchang Decoction(清肠汤,QCD)on ulcerative colitis(UC)mice and its effect on hepatocyte growth factor(HGF)/signal transducer and activator of transcription 3(STAT3)/nuclear factor kappa-B(NF-κB)pathway.Methods Forty C57BL/6 mice were divided into control group,dextran sulfate(DSS)group,sulfasalazine(SASP)group(100 mg/kg)and QCD group(18 g/kg).The mice in each group except the control group drank 2.5％DSS solu-tion freely for 7 days,and then replaced with normal drinking water.The normal control group and model group were given distilled water by gavage,and the other groups were given the corresponding drugs.The general condition and body weight of mice were ob-served every day.After 10 days,the mice were killed.The colon length was measured.The tissue morphology of colon tissue was analyzed.The concentrations of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1 β)and interleukin-6(IL-6)in co-lon tissue were measured by ELISA.The expression levels of HGF,p-STAT3 and p-NF-κB were detected by Western blot.Results QCD significantly increased the weight and colon length of mice,decreased the pathological score of colon,significantly reduced the concentrations of TNF-α,IL-1 β and IL-6,and reduced the expression levels of HGF,p-STAT3 and p-NF-κB in colon tissue.Conclusion QCD may treat DSS induced UC mice by regulating HGF/STAT3/NF-κB pathway.