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RFX1-induced transcriptional activation of FZD2 accelerates the progression of breast cancer
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- Author:
- No author available
- Journal Title:
- Military Medical Journal of Southeast China
- Issue:
- 3
- DOI:
- 10.3969/j.issn.1672-271X.2023.03.002
- Key Word:
- 乳腺癌;转录调节因子X1;FZD2;转录因子;breast cancer;regulator factor X1;FZD2;transcription factor
Abstract： 目的 探讨转录调节因子X1(RFX1)在乳腺癌中的表达及功能,探索其在乳腺癌中的调控机制.方法 通过实时荧光定量PCR检测乳腺癌及配对乳腺癌旁组织中RFX1的相对表达量;通过生物信息学、染色质免疫沉淀技术(ChIP)和荧光素酶实验验证转录因子RFX1的靶基因;通过Western blot、CCK8、Transwell和划痕实验明确RFX1对乳腺癌增殖、侵袭、转移和上皮-间质转化(EMT)的影响.结果 RFX1在乳腺癌组织中的表达高于癌旁正常组织(P＜0.01),其表达水平与乳腺癌分期、类型和淋巴结转移状态有关(P＜0.01).体外实验结果表明RFX1促进乳腺癌细胞的增殖、迁移、转移和EMT过程,差异具有统计学意义(P＜0.05).生物信息学方法预测RFX1靶向调控FZD2,过表达RFX1使FZD2启动子区转录活性增强(P＜0.01).挽救实验提示外源性下调RFX1后乳腺癌细胞的增殖、侵袭、迁移和上皮-间质转化被FZD2逆转,差异具有统计学意义(P＜0.05).结论 RFX1通过上调FZD2促进乳腺癌细胞的增殖、侵袭和转移.这一发现为乳腺癌的治疗提供了新的思路和靶点.
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