Abstract： Objective To analyze the distribution of cervical human papillomavirus(HPV)infection and genotype in healthy adult women in Simao District,Yunnan Province,to provide a scientific basis for the prevention of cervical HPV infection in women and vaccine development.Methods Cervical cytology samples were taken from healthy female subjects aged 18～45 years who participated in a clinical trial of HPV vaccine,and HPV genotype were detected by PCR-reverse dot hybridization.Descriptive method was used to analyze the HPV infection rate,genotype distribution,single and multiple infection distribution characteristics.Results Among 697 healthy female subjects aged 18～45 years,the overall cervical HPV infection rate was 20.80％.A total of 22 HPV genotypes were detected,and the main high-risk genotypes were 52(15.00％),16(1 1.00％),58(7.00％),53(6.00％)and 51(6.00％).The low-risk genotypes were 43(6.50％)and 81(5.50％).The HPV infection rate in the age group of 18～25 years was the highest(25.21％),and decreased with age(x2=4.490,P＞0.05),The main types of infection in all age groups were 52,16 and 43.The infection pattern was dominated by single infection(73.79％),and the genotypes were mainly HPV 52,16,58,59,56 and 43.The most severe cases of multiple infections were five major infections,of which double infection was dominant(73.68％),and the genotypes were HPV52 or 16 with other high-risk genotypes.The rate of multiple infections decreased significantly in the 36～45 years old group.Conclusion The cervical HPV infection rate of adult healthy women in Simao District was relatively high,and the main high-risk HPV genotypes were basically the same as those in China and other areas of Yunnan,but the main low-risk types were HPV43 and 81.