Abstract： Objective To investigate the pharmacodynamic material basis of American ginseng in their anti-lung cancer effect.Methods The UPLC-Q-Exactive MS technique was utilized to establish a quantitative fingerprint detection method for saponins in American ginseng.The drug efficacy was evaluated using a zebrafish xenotransplantation model.Partial least square correlation regression analysis was employed to analyze the active components of American ginseng that inhibit lung cancer.Results By analyzing the spectral and efficacy data and conducting partial least squares correlation regression analysis on 40 batches of American ginseng samples,it was observed that most saponins in American ginseng were positively correlated with inhibiting lung cancer progression.The order of drug efficacy contribution is as follows:malonyl ginsenoside Rb1>ginsenoside Rb1>acetyl ginsenoside Rb1>chikusetsusaponin Ib>malonyl ginsenoside Re>yesanchinoside B>malonyl ginsenoside Re1>ginsenoside Ro>panaxjaponicussaponin Ⅳa(VIP>1).Conclusion American ginseng saponins can inhibit the proliferation of A549 lung cancer cells in zebrafish,and the screened active ingredients can provide data support for further research and development of anti-lung cancer drugs.