Abstract： Objective To screen the differential expression of miRNA of endometrial cancer(EC)tissues,to provide a basis for further study of the correlation of miRNA with the development EC.Methods miRNA microarray was used to detect the differential expression of miRNA between human EC tissues and normal endometrial tissues.Real-time quantitative RT-PCR was performed to detect the differential expression of candidate miRNA obtained from microarray experiment.Results Compared with normal endometrial tissues,14 miRNA were upregulated,and 6 miRNA downregulated in EC tissues.The results of real-time quantitative RT-PCR were consistent with those of miRNA microarray.Conclusion The aberrant expression of miRNA in this study may be involved in the pathogenesis of EC.