Abstract： Objective To explore the the expression of VEGF,HIF-1 α and Sp1 in cancer tissue of children with oral cancer and its clinical significance.Methods A study was conducted on 63 children with oral cancer,all of whom underwent sur-gical treatment.During the surgery,tumor tissue and adjacent tissues were removed,and VEGF,HIF-1 α and Sp1 were detected u-sing immunohistochemistry,Compared the expression of VEGF,HIF-1 α and Sp1 in tumor tissues and adjacent tissues;And ana-lyzed the expression of VEGF,HIF-1 α and Sp1 and its relationship with clinical pathological features.Results Positive rate of the expression of VEGF,HIF-1 α and Sp1 in tumor tissues were higher than those in adjacent tissues,and the difference was sta-tistically significant(P＜0.05);The positive rate of VEGF in patients with lymph node metastasis,clinical stage Ⅲ～Ⅳ,low dif-ferentiation degree,and tumor diameter≥3 cm was higher than of patients without lymph node metastasis,stage Ⅰ～Ⅱ,high dif-ferentiation degree,and tumor diameter＜3 cm,with statistical significance(P＜0.05);The positive rate of HIF-1 α in patients with lymph node metastasis,clinical stages Ⅲ～Ⅳ,low differentiation,and tumor diameter≥3 cm was higher than that of patients without lymph node metastasis,stage Ⅰ～Ⅱ,high to medium differentiation,and tumor diameter＜3 cm,with a statistically sig-nificant difference(P＜0.05);The Sp1 positive rate in patients with lymph node metastasis,clinical stage Ⅲ～Ⅳ,low differentia-tion degree,and tumor diameter ≥ 3 cm was higher than that in patients without lymph node metastasis,stage Ⅰ～Ⅱ,high differ-entiation degree,and tumor diameter＜3 cm,with statistical significance(P＜0.05).Conclusion VEGF,HIF-1α、Sp1 is high-ly expressed in oral cancer tissue and is closely related to clinical staging,lymph node metastasis,and other factors.Clinical atten-tion should be paid to it in order to carry out targeted treatment work.