Abstract： Objective To analyze the therapeutic effect of 3D laparoscopic surgery in patients with differentiated thyroid cancer(DTC).Methods 96 patients with DTC were selected and randomly divided into 2 groups,each with 48 cases,using a random number table method.The control group underwent 2D laparoscopic surgery,while the observation group underwent 3D laparoscopic surgery,with an observation period of 3 months after surgery.Compare perioperative indicators,thyroid hormone lev-els,and complications between 2 groups.Results The intraoperative blood loss in the observation group[(16.23±2.41)ml]was lower than that in the control group[(23.49±3.78)ml],and the surgical time[(54.72±4.63)min]was shorter than that in the control group[(67.58±6.21)min],with statistical significance(P＜0.05);There was no statistically significant differ-ence in hospital stay and complications between the 2 groups(P＞0.05).There was no statistically significant difference(P＞0.05)in the levels of free triiodothyronine(FT3),free thyroxine(FT4),and thyroid stimulating hormone(TSH)between the 2 groups before and after surgery;But after surgery,the FT3 and FT4 of the 2 groups were lower than before surgery,and TSH was higher than before surgery,with statistical significance(P＜0.05).Conclusion Both 2D laparoscopic surgery and 3D laparo-scopic surgery can effectively treat DTC patients,but both have a certain impact on the patient's thyroid function.However,3D lap-aroscopic surgery has a shorter surgical time and less intraoperative bleeding,which is worthy of clinical promotion.