Abstract： Objective:To analyze the effects of assisted intestinal fluid infusion on anal function, gastrointestinal function and nutritional status of patients with low rectal cancer after terminal ileostomy.Methods:Eighty patients with low rectal cancer admitted to Zhejiang Jinhua Guangfu Cancer Hospital from July 2021 to January 2023 were selected as the study objects, and 40 patients performed radical operation and terminal ileostomy(control group), 40 patients performed radical operation and terminal ileostomy and intestinal fluid infusion (experimental group). The anal function indexes, gastrointestinal hormone indexes, intestinal mucosal barrier function indexes and nutritional status indexes were compared before and after operation between the two groups.Results:After operation for 3 months, the maximum systolic pressure of anal canal, rectal compliance, maximum resting pressure of anal canal, rectal bowel inclination sensory volume in the experimental group were higher than those in the control group: (169.72 ± 12.04) mmHg (1 mmHg = 0.133 kPa) vs. (160.42 ± 11.58) mmHg, (4.88 ± 0.17) mmHg vs. (4.20 ± 0.14) mmHg, (40.73 ± 4.31) mmHg vs. (36.05 ± 4.09) mmHg, (80.31 ± 8.27) ml vs. (72.53 ± 7.39) ml, there were statistical differences ( P<0.05). After operation for 9 d, the levels of gastrin (GAS), motilin (MTL), prealbumin (PA), albumin (Alb) and hemoglobin (Hb) in the experimental group were higher than those in the control group: (260.59 ± 31.57) ng/L vs. (224.61 ± 25.01) ng/L, (91.73 ± 10.58) ng/L vs. (65.65 ± 7.01) ng/L, (167.89 ± 18.14) mg/L vs. (152.34 ± 16.01) mg/L, (24.58 ± 2.89) g/L vs.(21.49 ± 2.47) g/L, (79.84 ± 8.67) g/L vs. (70.59 ± 8.01) g/L, there were statistical differences ( P<0.05). The incidence of malnutrition in the experimental group was lower than that in the control group: 10.00%(4/40) vs. 27.50%(11/40), there was statistical difference ( χ2 = 4.02, P<0.05). Conclusions:The use of intestinal fluid infusion as an adjuvant therapy after distal ileostomy in patients with low rectal cancer can improve anal function, gastrointestinal function, and nutritional status.