Abstract： Objective To provide reproductive counseling and guidance for a pregnant woman who previously had a child with comprehensive developmental delays.Methods The proband has undergone a child development assessment,brain MRI scan,and whole-exome sequencing for the family.Based on the AC MG/AMP variant classification guidelines and supplementary recommendations from ClinGen,the proband's genetic report was re-evaluated.Results Whole-exome sequencing of the family identified a novel variant of uncertain significance in the TUBB3 gene(NM_006086.4),c.86G＞A(p.Gly29Asp).After re-evaluating the pathogenicity of the variant,it was classified as likely pathogenic.This variant has not been previously reported in the literature.Considering the clinical phenotype,variant pathogenicity,pathogenic mechanism,inheritance pattern,and after a multidisciplinary team consultation,the proband was considered to have cortical dysplasia with other brain malformations type I.Conclusion Re-evaluating genetic reports with unclear clinical significance can improve diagnostic yield and provide valuable guidance for reproductive counseling.