Abstract： Objective By simulating the nursing skills,management experience and precautions close to the clinical perioperative period,a standardized and regulated postoperative management model of mouse cecal ligation and perforation(CLP)sepsis animal model was established,and its influence on the model establishment was evaluated.Methods BALB/C male mice were randomly divided into the control group and the experimental group,with 15 mice in each group,and the CLP model was made(cecal ligation length of 1 cm,and 12 G needle penetrated 2 holes).The mice in the two groups were given routine feeding before operation,and the mice in the experimental group were treated with heat preservation,fluid rehydration and wound care after operation,while the control group mice were not treated after operation.The body weight,feed intake,animal status and survival rate 7 days after operation of mice in the two groups were recorded.Results After modeling,the body weight recovery,feed intake and animal status of mice in the experimental group were significantly better than those in the control group(P<0.05).At the same time,the 7-day survival rate of mice in the experimental group was 80.0%,which was significantly higher than that of 53.3%in the control group(P<0.05).Conclusion Applying perioperative management experience to the establishment of mice sepsis model is helpful to improve the quality and efficiency of animal experiments,which can avoid the unplanned death of model animal caused by postoperative accidents,and interfere with the judgment of the results.At the same time,it will be closer to the treatment and management measures of clinical sepsis patients,and provide reference for the establishment and application of standardized sepsis animal model.