Abstract： Objective:To investigate the impact of intravertebral analgesia on the outcome of vaginal delivery in pregnant women with Group B streptococcus (GBS).Methods:A total of 212 pregnant women with GBS were retrospectively selected from January 2022 to December 2022 in the Affiliated Suzhou Hospital of Nanjing Medical University, 106 pregnant women with intraspinal analgesia were selected as the study group and 106 pregnant women without intraspinal analgesia were selected as the control group. The perinatal outcomes of pregnant women and newborns in the two groups were compared.Results:There were no significant differences in age, BMI, gravida, para and gestational weeks between the two groups (P>0.05). The postpartum blood loss [(284.34±67.13)ml vs (262.78±80.45)ml], the first stage of labor [(453.62±149.03)min vs (411.22±136.25)min], the second stage of labor [(46.11±19.33)min vs (40.59±17.22)min] and intrapartum fever [21(19.81%) vs 6(5.66%)] in the study group were higher than those in the control group, the differences were statistically significant (P<0.05). There were no significant differences in the third stage of labor, lateral perineal incision, vaginal midwifery, cesarean section and postpartum hemorrhage between the two groups (P>0.05). There were no significant differences in neonatal weight, Apgar score (1 min, 5 min), cord blood pH, BE, neonatal asphyxia and NICU admission between the two groups (P>0.05).Conclusions:Intravertebral analgesia in pregnant women with GBS in labor can significantly relieve pain, and the first and second stages of labor may be prolonged. However, it does not affect the perinatal outcome of mother and newborns, and is a safe and effective way of labor analgesia.