Abstract： Objective:To explore whether Corona Virus Disease 2019 (COVID-19) has any impact on human centrifuge training of flying cadets and to provide references to the human centrifuge training for the recovered flying personnel by comparing the changes of flying cadets′ G-tolerance and physiological indicators in human centrifuge training.Methods:Twelve flying cadets who underwent the current batch of human centrifuge training in 2022 were selected and divided into rehabilitation group and control group according to whether they had been infected with COVID-19, with 6 in each group. The rapid G onset rate (ROR) and gradual G onset rate (GOR) were respectively chosen for human centrifuge test. At the same time, the changes of their heart rate and respiratory rate during the tests were recorded, and the G-tolerance situations of the subjects were collected.Results:The average relaxed G-tolerance under GOR (GOR tolerance 1) was (4.2±0.8) G in rehabilitation group, and their average anti-G straining maneuver aided G-tolerance under GOR (GOR tolerance 2) was (5.7±0.6) G. Compared with that of the control group, there was no significant difference in GOR tolerance 1 and GOR tolerance 2 (both P>0.05). There was no significant changes in heart rate and respiratory rate in ROR and GOR human centrifuge tests between 2 groups (all P>0.05). Conclusions:COVID-19 didn't impact the recovered flying cadets' performance in human centrifuge training, as well as the anti-G straining maneuver effect and cardiovascular regulation.