Abstract： Objective To investigate the effects of C-Maf inducing protein(CMIP)on doxorubicin resistance in human colorectal cancer.Methods Sixty cases of colorectal cancer tissues and 60 cases of adjacent normal tissues were collected from Huaibei People's Hospital from January 2021 to December 2022.The expression of CMIP in colorectal cancer tissues was detected by immunohistochemistry,and the correlation between CMIP and clinicopathological characteristics of colorectal cancer was analyzed.Proliferation and migration of human colorectal cancer HCT116 and HT29 cell lines were assessed using CCK8 assay and cell scratch assay,respectively.Potential interacting targets of CMIP were predicted using the catRAPID omics v2.0 website.The GO function and KEGG pathway enrichment analysis of the target genes of CMIP were performed by Metascape website.Results The expression of CMIP was upregulated in human colorectal cancer tissues,and was correlated with the clinicopathological features of colorectal cancer.Overexpression/knockdown of CMIP promoted/inhibited proliferation and migration of colorectal cancer cells,as well as enhanced/resisted adriamycin resistance in these cells.A total of 205 CMIP-related mRNAs were found by catRAPID omics v2.0 database.GO and KEGG analysis results indicated that CMIP may mediate doxorubicin resistance in colorectal cancer through multiple biological processes and signaling pathways.Conclusion CMIP can facilitate the development of colorectal cancer and its resistance to doxorubicin.However,further investigation is required to elucidate its specific mechanism.