Abstract： Ovarian cancer is the most threatening disease among gynecological tumors,with a variety of causes and com-plex mechanisms working together to promote the disease.It can mediate the entire process of tumor cell proliferation and metastasis by its unique microenvironment pattern.The clinical treatment of ovarian cancer at present is mainly based on cytoreductive surgery combined with platinum-based chemotherapeutic drugs,but the side effects and multi-drug resis-tance of drugs have led to a high recurrence rate.In order to explore new methods with fewer side effects or to reduce the side effects of traditional chemotherapeutic drugs,researchers have tried to use Chinese medicine combined treatment in the process of tumor diagnosis and treatment.It has been confirmed that the single components of Chinese medicine can slow down the tumor progression by regulating tumor cells,immune cells,hypoxia-inducible factors,vascular endothelial growth factors,inflammatory mediators,and corresponding signaling pathways in the microenvironment,thereby exerting an-ti-cancer effects.Berberine,a common active component found in many herbal medicines,has tumor cell sensitivity,which enables it to exert anti-tumor effects through different mechanisms,reversing platinum resistance and reducing the recur-rence rate of ovarian cancer.This paper reviewed the literature and summarized the experimental studies on the effects of berberine on the tumor microenvironment.It was concluded that berberine could inhibit the proliferation,invasion,and mi-gration of tumor cells by regulating nuclear transcription factor-κB(NF-κB)and Janus kinase/signal transducer and activa-tor of transcription(JAK/STAT),improving the inflammatory and hypoxic microenvironment,and inducing cell apoptosis and cell cycle arrest.At the same time,this paper integrated and discussed on the action sites and mechanisms of berberine in the ovarian cancer microenvironment,intending to extend new ideas for frontier drug research and provide theoretical ba-sis for clinical treatment and prognosis of ovarian cancer.