目的 探讨毛发鼻指(趾)综合征1型相关基因(tricho-rhino-pharyngeal syndrome type 1,Trps1)在冷保存再灌注损伤(cold preservation reperfusion injury,CPRI)诱导人肝内胆管上皮细胞(human intrahepatic biliary epithelial cells,HIBECs)上皮-间质转化(epithelial-mesenchymal transition,EMT)中的作用.方法 体外模拟CPRI处理HIBECs检测上皮标志物E-cadherin、CK19,间质标志物Vimentin、α-SMA及目标基因Trps1的表达.用Trps1腺病毒或Trps1 siRNA载体转染HIBECs后,重复CPRI诱导实验,探讨Trps1对HIBECs EMT的调控作用.结果 随着冷保存时间的延长,上皮标志物E-cadherin、CK19 mRNA和蛋白表达持续减少(P=0.018,0.011;P=0.039,0.044),间质标志物Vimentin、α-SMA表达持续增加(P=0.002,0.026;P=0.035,0.016);冷保存12 h,细胞内Trps1 mRNA和蛋白表达增加(P=0.018,0.031),其后随冷保存时间延长持续减少(P=0.026,0.009).相关分析显示在该过程中,Trps1与上皮标志物表达正相关(r=0.981,0.913;P =0.000,0.002),而与间质标志物表达负相关(r=-0.711,-0.847;P=0.009,0.005).Trps1腺病毒转染HIBEC后,重复上述CPRI诱导实验,E-cadherin表达增加(P =0.002,0.000),Vimentin表达减少(P=0.000,0.017),CPRI诱导HIBECs EMT效应

作者：程哲；田驹；郑树国

来源：第三军医大学学报 2014 年 36卷 8期