Introduction::Myiasis is an infestation of the tissues and organs of living vertebrates and humans by fly larvae, usually those belonging to the Calliphoridae family. The larvae feed on the host’s necrotic or living tissue.Case presentation::On mucocutaneous examination, the patient had multiple sinus tracts covered with purulent necrotic slough, and cylindrical maggots were crawling out of and around the areas of slough. The patient was diagnosed with wound myiasis caused by Cochliomyia hominivorax. Turpentine oil flushes and oral ivermectin were prescribed. Discussion::Cutaneous myiasis can be papular, pustular, furuncular, and nodular. It is more common in people who have poor hygiene and intimate contact with domestic animals, such as people living in rural areas and tropical/subtropical countries. The chief aim of management is to remove the maggots as soon as possible, kill them to prevent maturation, promote wound healing, and prevent development of secondary complications.Conclusion::In the current case, the patient’s poor nursing and personal care and low socioeconomic condition predisposed her to secondary wound infection and maggot infestation. Such cases require prompt care to prevent secondary infection and septicemia.

目的:观察医用五谷虫对糖尿病足的疗效，并分析其可能的作用机制。方法:选取2018年7月至2019年9月大连医科大学附属第二医院Wagner分级1~2级糖尿病足溃疡患者60例，按随机数字表法分为对照组（采用常规方法治疗）和试验组（采用常规方法联合医用五谷虫治疗），每组30例。试验组患者于治疗前和治疗第4天采用聚合酶链反应法检测创面Notch 1 mRNA表达量。比较两组治疗后21 d创面愈合率。结果:试验组痊愈率明显高于对照组[100.00%（30/30）比76.67%（23/30）]，差异有统计学意义（ χ2 = 7.925， P<0.01）。试验组治疗前和治疗第4天创面Notch 1 mRNA表达量比较差异无统计学意义（0.96 ± 0.08比0.93 ± 0.08， t = 1.545， P>0.05）。 结论:医用五谷虫对于Wagner分级1~2级糖尿病足溃疡具有明显疗效，其作用机制可能与Notch 1信号通路无关。

目的 探讨低温冷藏时间对医用级丝光绿蝇幼虫存活率及发育的影响.方法 采集室内传代培养的丝光绿蝇蝇卵,经消毒后孵化出1日龄、2日龄、3日龄幼虫,再次消毒后于6℃保存,计算冷藏保存第1 ~7 天各日龄幼虫的存活率,存活率>50%时取存活的蛆虫于常温下继续培养2 d,记录蛆虫的存活率及体重.结果 1日龄蛆虫冷藏第1~2天的存活率均>50% ,且高于冷藏第3~7天的蛆虫(均P<0. 05);2日龄蛆虫冷藏第1~3天的存活率均>50% ,且高于冷藏第4~7天的存活率(均P<0. 05);3日龄的蛆虫第1~7天的存活率均>50% ,且冷藏第6~7天存活率均低于冷藏第1~4天的存活率(均P<0. 05). 1日龄蝇蛆冷藏1 d后再培养,2日龄蝇蛆冷藏1~2 d后再培养,3日龄蝇蛆冷藏1~5 d后再培养,其存活率均达80%以上,且生长发育情况良好.结论 1日龄、2日龄、3日龄医用级蛆虫相应的适宜冷藏保存时间分别为1 d、2 d和5 d.

The late Dr. W. S. Baer, who advocated the use of live maggots for chronic osteomyelitis, stated that when America first entered the World War in 1917 the mortality of compound fractures of the femur was about eighty per cent (1). In his opinion this heavy death rate was due to want of proper first aid spIinting and bad management of the transportation. But I think the method of treatment employed at the time must have been responsible to some extent. Evidently this subject is worthy of serious discussion.