OBJECTIVE:To study the anti-inflammatory and anti-tussive effects of Qingfei Dayuan granules(清肺达原颗粒,QFDY),and to evaluate the acute and sub-chronic toxicity of QFDY.METHODS:Anti-inflammatory effects were evaluated by murine model of xylene induced ear edema in mice.Ear swelling degree was calculated and tumor necrosis factor-α,interleukin-1β and interleukin-6 were determined.Anti-tussive evaluations were carried out in the mouse cough model induced by ammonia liquor.Latent period cough and number of cough within 3 min were counted.In acute toxicity study,the rats were randomly divided into test group and solvent control group.Body weighs,food intakes and general clinical signs were monitored.In the sub-chronic toxicity study,QFDY was administered to rats at 0,4,8 and 16 g/kg per day for 28 and 30 d of post treatment was conducted.Mortalities,clinical signs,body weight changes,food intakes,ophthalmological examinations,hematological parameters,biochemical indicators,electrolyte indicators,urinalyses and histopathological examinations were monitored.RESULTS:QFDY significantly inhibited the development of ear edema in anti-inflammatory assay and decreased cough frequency caused by ammonia liquor.The results presented a dose-effect relationship.In acute toxicity study,no abnormality exhibited at dose of 24.0 g/kg per day during the 14-d observation period.In the sub-chronic toxicity study,higher reticulocyte count,lymphocyte and lower Cl-,blood urea nitrogen were analyzed compared with the solvent control group.But the differences were considered to be incidental and not clinically toxic.Obvious dose-effect relationship of urine color was observed,and the three test groups at the end of the experiments resulted in significant increase in urobilinogen,bilirubin,ketone body and urine leukocyte.However,all the positive indicators returned to normal in the recovery period.Therefore,no toxicological changes were found during the study period.CONCLUSION:QFDY showed significant anti-inflammatory and anti-tussive effects in mice.The lethal dose(LD50)of per oral QFDY in rats was estimated to be more than 24.0 g/kg per day and the no observed adverse effect level was over 16 g/kg per day,which suggested that QFDY is relatively safe for oral medication at the present dose on rats.Our experimental results provide a reference for the further development and research of QFDY.

目的 探究抗菌药物联合痰热清注射液治疗肺癌患者化疗后继发肺部感染的临床疗效观察.方法 选取2014年1月-2016年12月医院收治的120例肺癌患者化疗后继发肺部感染的患者作为研究对象,将患者分为试验组和对照组,每组60例;对照组采用抗菌药物联合治疗,试验组在对照组的基础上加用痰热清注射液治疗,治疗两个疗程,比较两组患者临床指标及有效率.结果 治疗后,试验组治疗总有效率为96.67%,高于对照组的86.67%(P<0.05);试验组的机体功能状态改善情况的总有效率为98.33%,高于对照组的85.00%(P<0.05);试验组临床症状消失的时间少于对照组(P<0.05);试验组血清MMP-9、TNF-α和IL-8的水平下降,且低于对照组(P<0.05);试验组的住院时间少于对照组(P<0.05);试验组未发生二重感染,对照组的二重感染的感染率为4.14%(P<0.05).结论 痰热清注射液与抗菌药物联合治疗肺癌患者化疗后的继发肺部感染,疗效显著,发挥了较好的止咳、抗炎、化痰的功效.

目的 比较太白贝母(野生、栽培)与松贝、 青贝、 炉贝生物碱大孔树脂纯化物的镇咳、 抗炎药效学差异,为其临床应用和进一步开发利用提供依据.方法 通过回流法提取,大孔吸附树脂纯化不同品种川贝母总生物碱;紫外分光光度法测定川贝母总生物碱含量.比较5种不同品种川贝母生物碱(2.3 mg·kg-1)对氨水致小鼠咳嗽及二甲苯致小鼠耳肿胀的镇咳、 抗炎药效学差异.对不同品种川贝母生物碱镇咳、 抗炎实验结果进行聚类分析.结果 经大孔吸附树脂纯化后,各川贝母样品生物碱含量在51.93％～68.38％之间.太白贝母(野生)、 太白贝母(栽培)、 松贝、 青贝、 炉贝生物碱均可明显延长小鼠咳嗽潜伏期;太白贝母(野生)、 松贝、 青贝、 炉贝生物碱可明显降低小鼠2 min咳嗽次数.青贝、炉贝生物碱可使二甲苯致小鼠耳肿胀率明显降低,而太白贝母(野生、 栽培)抗炎效果不明显.聚类分析表明,松贝、 青贝、 炉贝数据聚为一类,再与太白贝母(野生)聚集,而与太白贝母(栽培)分离.结论 太白贝母与松贝、 青贝、 炉贝生物碱一样具有镇咳作用,但抗炎效果不明显,其生物碱组成可能与松贝、 青贝、 炉贝有一定差异.

目的 探讨布地奈德对呼吸道合胞病毒(RSV)感染相关喘息患儿血清炎性因子水平的影响.方法 选择2015年8月至2017年8月湖南中医药大学第一附属医院儿科收治的RSV感染相关喘息患儿98例,按照随机数字表法分为对照组与观察组,各49例.对照组患儿给予常规拍背吸痰、吸氧、补液、平喘、止咳联合抗病毒类药物治疗,观察组在对照组基础上联合布地奈德混悬液治疗.比较2组患儿治疗2周后的临床疗效;治疗前和治疗2周后检测2组患儿肺功能,记录潮气量、达峰容积比(VPTEF/VE)以及达峰时间比(tPTEF/tE);采用放射免疫吸附法检测血清白细胞介素4(IL-4)、IL-6、IL-10和C反应蛋白含量.结果 治疗2周后,观察组临床总有效率显著高于对照组[95.9％ (47/49)比81.6％ (40/49)],差异有统计学意义(P＜0.05).治疗前,2组患儿肺功能相关指标水平比较差异均无统计学意义(均P＞0.05).治疗后,2组患儿潮气量、VPTEF/VE以及tPTEF/tE均高于治疗前,且观察组高于对照组[(8.7±2 6)ml/kg比(7.4±2.1) ml/kg,(29±7)％比(24±6)％,(30±6)％比(23±4)％],差异均有统计学意义(均P＜0.05).治疗前,2组患儿血清炎性因子水平比较差异均无统计学意义(均P ＞0.05).治疗后,2组患儿血清IL-4、IL-6及C反应蛋白水平均低于治疗前,且观察组低于对照组[(7.6±1.4)ng/L比(12.3±2.4) ng/L,(15.6±2.4)ng/L比(21.3±5.4) ng/L,(6.4±1.1)mg/L比(9.0±1.2) mg/L];IL-10水平均高于治疗前,且观察组高于对照组[(14.0±3.0) ng/L比(9.4±1.9) ng/L],差异均有统计学意义(均P＜0.05).结论 常规治疗联合布地奈德治疗RSV感染相关喘息效果显著,能够有效抑制气道炎症.

目的：探讨稳定期COPD合并便秘患者引入综合干预对其生活质量及症状改善的影响，并为这类群体的优质护理积累循证经验。方法采用方便抽样法于2012年7月—2015年2月选择稳定期COPD合并便秘患者，按照入院的先后顺序分为研究组和对照组，每组各42例。其中对照组实施常规的化痰、止咳、抗炎治疗，而研究组则在对照组干预的基础上实施综合干预。两组患者在干预前1d及干预后第4周末均接受圣乔治呼吸问卷( SGRQ)调查，并记录两组患者便秘症状改善情况。结果两组患者治疗前SGRQ评分差异无统计学意义(P>0.05)；治疗后第4周末，研究组呼吸症状、活动受限、疾病影响及总分评分均低于对照组，差异有统计学意义(P<0.05)。研究组便秘症状改善总有效率高于对照组，差异有统计学意义(P<0.01)。结论综合干预有助于改善稳定期COPD合并便秘者的便秘症状，能提高其生活质量。