目的 了解生育三烯酚对小鼠免疫功能的影响.方法 根据《保健食品检测与评价技术规范》的要求,设立3个剂量组(50、75和100mg/kg·BW)和1个阴性对照组.经过30天的口服给与不同剂量生育三烯酚后,比较小鼠的免疫器官脏器系数、细胞免疫功能、体液免疫功能、单核-巨噬细胞功能和自然杀伤细胞(natural killer cell,NK)活性的变化.结果 与对照组小鼠半数溶血值(half value of hemolysin,HC50)(223.0±6.7)相比较,75 和 100mg/kg·BW 剂量组的 HC50 值(238.0±7.3,245.2±7.8)明显升高,差异均有统计学意义(均P＜0.05);75 mg/kg·BW组小鼠抗体生成细胞数[(44.4±5.7)×103个]较对照组[(36.7±6.2)×103个]增加,差异有统计学意义(P＜0.05);生育三烯酚促进刀豆蛋白A(concanavalin A,ConA)诱导后脾淋巴细胞转化能力75和100 mg/kg·BW组(0.249±0.042、0.256±0.033)明显高于对照组(0.183±0.064),差异均有统计学意义(均P＜0.05);100mg/kg·BW组小鼠在绵羊红细胞攻击前后左后跖厚度差值[(0.60±0.14)mm]明显高于对照组[(0.47±0.08)mm],差异有统计学意义(P＜0.05);75和100 mg/kg·BW组NK细胞活性分别为24.8±4.8、26.4±7.8,明显高于对照组(14.2±4.3),差异均有统计学意义(均P＜0.05).结论 生育三烯酚对小鼠免疫力有增强的作用.

目的 建立加速溶剂萃取(ASE)结合亲水液相色谱(HILIC)柱分离测定坚果中8种生育酚异构体的方法.方法 2 g坚果样品经ASE提取富集后,用BEH Amide色谱柱(150 mm×3.0 mm,1.7 μm)分离,以90％正己烷,10％叔丁基甲基醚-四氢呋喃-甲醇(20∶ 1∶0.1,V/V)作为流动相等度洗脱,荧光检测器激发波长294 nm,发射波长328 nm检测,外标法定量.结果 4种生育酚在0.5～ 80.0 μg/ml、4种生育三烯酚在0.5～ 30.0 μg/ml之间具有良好的线性(r2 ＞0.998),方法检出限在0.032～0.070 mg/kg之间,方法定量限范围0.096 ～ 0.210 mg/kg,三个水平加标回收率在87.5％ ～ 114.2％之间,6次重复测定相对标准偏差(RSD)均≤12％.结论 本方法具有良好的灵敏度、回收率和重复性,适用于坚果中8种生育酚异构体的含量分布的测定.

Objective: Tocotrienols (T3s) have been hypothesized to have greater antioxidant capacity than tocopherols (Ts) due to differences in biokinetics that affect their absorption and function.The present trial compares the antioxidant effectiveness following postprandial challenge of two different doses of α-T or palm T3-rich fraction (TRF) treatments and evaluates their dose-response effects on antioxidant status.Methods: Ten healthy volunteers were given four different doses of vitamin E formulations (268 mg α-T,537 mg α-T,263 mg TRF or 526 mg TRF) in a cross-over postprandial trial.Blood was sampled at 0,2,4,5,6 and 8 hours after meal consumption and plasma antioxidant status including total glutathione,super-oxide dismutase,malondialdehyde (MDA),ferric reducing antioxidant potential and trolox-equivalent antioxidant capacity,was analyzed.Results: Supplementation with the different doses of either α-T orTRF did not significantly improve overall antioxidant status.There was no significant difference in overall antioxidant status among treatments at the different doses compared.However,a significant dose-response effect was observed for plasma MDA throughout the 8-hour postprandial period.MDA was significantly lower after the 537 mg α-T treatment,compared to the 268 mg α-T treatment;it was also lower after the 526 mg TRF treatment compared to the 263 mg TRF treatment (P ＜ 0.05).Conclusion: T3 and α-T demonstrated similar antioxidant capacity,despite markedly lower levels of T3 in blood and lipoproteins,compared to α-T,